Quantitative Label-Free Phosphoproteomics Strategy for Multifaceted Experimental Designs
نویسندگان
چکیده
منابع مشابه
Quantitative Label-Free Phosphoproteomics Strategy for Multifaceted Experimental Designs
Protein phosphorylation is a critical regulator of signaling in nearly all eukaryotic cellular pathways and dysregulated phosphorylation has been implicated in an array of diseases. The majority of MS-based quantitative phosphorylation studies are currently performed from transformed cell lines because of the ability to generate large amounts of starting material with incorporated isotopically ...
متن کاملEvaluation of different phospho-tyrosine antibodies for label-free phosphoproteomics.
BACKGROUND Mass spectrometry based phosphoproteomics emerged as advantageous approach for the analysis of tyrosine phosphorylation on proteins and tyrosine kinase signaling. Immunoaffinity purification is required for comprehensive analysis. Here we compared the performance of two antibodies for label-free phosphotyrosine-based phosphoproteomics. METHODS Phosphopeptide immunoprecipitation of ...
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Inhalational anthrax is caused by spores of the bacterium Bacillus anthracis (B. anthracis), and is an extremely dangerous disease that can kill unvaccinated victims within 2 weeks. Modern antibiotic-based therapy can increase the survival rate to ∼50%, but only if administered presymptomatically (within 24-48 h of exposure). To discover host signaling responses to presymptomatic anthrax, label...
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Hyperactivated RAS drives progression of many human malignancies. However, oncogenic activity of RAS is dependent on simultaneous inactivation of protein phosphatase 2A (PP2A) activity. Although PP2A is known to regulate some of the RAS effector pathways, it has not been systematically assessed how these proteins functionally interact. Here we have analyzed phosphoproteomes regulated by either ...
متن کاملQuantitative Research Designs: Experimental
The ideas used in experiments today were mostly in place by the first few decades of the 20th century. The procedures of comparing groups, assigning individuals to treatments, and statistically analyzing group comparisons had been developed by 1940. During the 1960s, the types of experimental designs were identified and the strengths (e.g., control over potential threats) of these designs speci...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2011
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac200213b